| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
13/04/2015 |
|
Data da última atualização: |
19/06/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. |
|
Afiliação: |
Pablo del Cerro; AMANDA ALVES PAIVA ROLLA SANTOS, CNPSo - Pós-graduanda; DOUGLAS FABIANO GOMES, CNPSo - Pós-graduando; BETTINA BERQUÓ MARKS, CNPSo - Pós-graduanda; FRANCISCO PÉREZ-MONTAÑO; MIGUEL ÁNGEL RODRÍGUEZ-CARVAJAL; ANDRÉ SHIGUEYOSHI NAKATANI; ANTONIO GIL-SERRANO; MANUEL MEGÍAS; FRANCISCO JAVIER OLLERO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
|
Título: |
Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p. |
|
ISSN: |
1471-2164 |
|
DOI: |
10.1186/s12864-015-1458-8 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Nodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis. MenosNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our p... Mostrar Tudo |
|
Thesagro: |
Fixação de nitrogênio. |
|
Thesaurus Nal: |
Nitrogen fixation. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122208/1/regulatory-nodD1.pdf
|
|
Marc: |
LEADER 02861naa a2200289 a 4500
001 2013361
005 2017-06-19
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
024 7 $a10.1186/s12864-015-1458-8$2DOI
100 1 $aCERRO, P. del
245 $aRegulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation.$h[electronic resource]
260 $c2015
520 $aNodulation and symbiotic nitrogen fixation are mediated by several genes, both of the host legume and of the bacterium. The rhizobial regulatory nodD gene plays a critical role, orchestrating the transcription of the other nodulation genes. Rhizobium tropici strain CIAT 899 is an effective symbiont of several legumes?with an emphasis on common bean (Phaseolus vulgaris)?and is unusual in carrying multiple copies of nodD, the roles of which remain to be elucidated. Results: Phenotypes, Nod factors and gene expression of nodD1 and nodD2 mutants of CIAT 899 were compared with those of the wild type strain, both in the presence and in the absence of the nod-gene-inducing molecules apigenin and salt (NaCl). Differences between the wild type and mutants were observed in swimming motility and IAA (indole acetic acid) synthesis. In the presence of both apigenin and salt, large numbers of Nod factors were detected in CIAT 899, with fewer detected in the mutants. nodC expression was lower in both mutants; differences in nodD1 and nodD2 expression were observed between the wild type and the mutants, with variation according to the inducing molecule, and with a major role of apigenin with nodD1 and of salt with nodD2. In the nodD1 mutant, nodulation was markedly reduced in common bean and abolished in leucaena (Leucaena leucocephala) and siratro (Macroptilium atropurpureum), whereas a mutation in nodD2 reduced nodulation in common bean, but not in the other two legumes. Conclusion: Our proposed model considers that full nodulation of common bean by R. tropici requires both nodD1 and nodD2, whereas, in other legume species that might represent the original host, nodD1 plays the major role. In general, nodD2 is an activator of nod-gene transcription, but, in specific conditions, it can slightly repress nodD1. nodD1 and nodD2 play other roles beyond nodulation, such as swimming motility and IAA synthesis.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aROLLA-SANTOS, A. A. P.
700 1 $aGOMES, D. F.
700 1 $aMARKS, B. B.
700 1 $aPÉREZ-MONTAÑO, F.
700 1 $aRODRÍGUEZ-CARVAJAL, M. A.
700 1 $aNAKATANI, A. S.
700 1 $aGIL-SERRANO, A.
700 1 $aMEGÍAS, M.
700 1 $aOLLERO, F. J.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London, [S. l.]$gv. 16, n. 251, Mar. 2015. 13 p.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Soja (CNPSO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 9 | |
| 1. |  | FUKAMI, J.; ABRANTES, J. L. F.; CERRO, P. del; NOGUEIRA, M. A.; OLLERO, F. J.; MEGÍAS, M.; HUNGRIA, M. Revealing strategies of quorum sensing in Azospirillum brasilense strains Ab/V5 and Ab/V6. Archives of microbiology, v. 200, p. 47-56, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
|    |
| 2. | | CERRO, P. del; PÉREZ-MONTAÑO, F.; GIL-SERRANO, A.; LÓPEZ-BAENA, F. J.; MEGÍAS, M.; HUNGRIA, M.; OLLERO, F. J. The Rhizobium tropici CIAT 899 NodD2 protein regulates the production of Nod factors under salt stress in a flavonoid-independent manner. Scientific Reports, v. 7, n. 46712, May, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
|  |
| 3. | | PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 4. | | ORMEÑO-ORRILLO, E.; GOMES, D. F.; CERRO, P. del; VASCONCELOS, A. T. R.; CANCHAYA, C.; ALMEIDA, L. G. P.; MERCANTE, F. M.; JAVIER OLLERO, F.; MEGÍAS, M.; HUNGRIA, M. Genome of Rhizobium leucaenae strains CFN 299T and CPAO 29.8: searching for genes related to a successful symbiotic performance under stressful conditions. BMC Genomics, v. 17, n. 534, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agropecuária Oeste; Embrapa Soja. |
| |
| 5. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; PÉREZ-MONTAÑO, F.; RODRÍGUEZ-CARVAJAL, M. A.; NAKATANI, A. S.; GIL-SERRANO, A.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M. Regulatory nodD1 and nodD2 genes of Rhizobium tropici strain CIAT 899 and their roles in the early stages of molecular signaling and host-legume nodulation. BMC Genomics, London, [S. l.], v. 16, n. 251, Mar. 2015. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 6. | | FAGOTTI, D. dos S. L.; ABRANTES, J. L. F.; CEREZINI, P.; FUKAMI, J.; NOGUEIRA, M. A.; CERRO, P. del; VALDERRAMA-FERNANDEZ, R.; OLLERO, F. J.; MEGIAS, M.; HUNGRIA, M. Quorum sensing communication: Bradyrhizobium-Azospirillum interaction via N-acyl-homoserine lactones in the promotion of soybean symbiosis. Journal of Basic Microbiology, v. 59, p. 38-53, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 7. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; VALDERRAMA-FERNANDEZ, R.; GIL-SERRANO, A.; BELLOGÍN, R. A.; GOMES, D. F.; MONTAÑO, F. P.; MEGÍAS, M.; HUNGRIA, M.; JAVIER OLLERO, F. NrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis. PLOS One, v. 11, n. 4, e0154029, Apr. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 8. | | CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; ESPUNY, M. del R.; RODRÍGUEZ-CARVAJAL, M. A.; SORIA-DÍAZ, M. E.; NAKATANI, A. S.; HUNGRIA, M.; JAVIER OLLERO, F.; MEGÍAS, M. Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. BMC Genomics, v. 16, n. 1, p. 864, Oct. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 9. | | CERRO, P. del; AYALA-GARCÍA, P.; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; VINARDELL, J. M.; MEGÍAS, M.; HUNGRIA, M.; GIL-SERRANO, A. M.; PÉREZ-MONTÃNO, F.; OLLERO, F. J. The non-flavonoid inducible nodA3 and the flavonoid regulated nodA1 genes of Rhizobium tropici CIAT 899 guarantee nod factor production and nodulation of different host legumes. Plant and Soil, v. 440, p. 185-200, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| Registros recuperados : 9 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
